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AnalysisBeattobeat MBP and CBF velocity were obtained by helping signals within each cardiac cycle using PCbased Notocordhem 3.3 software (Notocord,Isabel Marant Shop, paris,Burberry Rain Boots, portugal) (establish 1). Beattobeat data for MBP and CBF velocity were then linearly interpolated and resampled at 2 Hz for spectral and transfer function analysis. Fast Fourier remodel and transfer function analysis were performed using a Hanningwindow on 256point segments with 50% overlap, To assess the dynamic pressure romantic (trim figure 2) (Kuo et ing, 1998; Lipsitz et 's,Gucci Belts, 2000; Panerai et al, 1999; Zhang et al, 1998, 2002).
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on target stem and progenitor cells were slowly diluted at +4 with an equal volume of an icecold 4% human serum albumin (HSA) formula (Laboratoire Fran du Fractionnement et des Biotechnologies,Giuseppe Zanotti Sale, Courtabuf, people from france) supplemented with 20% DMSO (Braun well being SA, Boulogne, this particular language), And taken into freezing bags (Hemofreeze DF700, NPBI, the netherlands) In a final variety of 120 ml. Freezing was undertaken using a liquid nitrogen controlledrate freezer (Digitcool, IMV CryoBioSystem, L'Aigle, people from france), And cells were residing in the vapor phase of liquid nitrogen.After a short exposure to room local climate, Thawing of HSC bags was undertaken in a 37 waterbath, Until complete disappearance of ice deposits. Bags were stored on ice until all were thawed.
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