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CAIR1/BAG3 binds within PLC SH3 domain: PLC can talk with partner proteins through multiple domains,Louis Vuitton Bags, like its two SH2 domains and an SH3 domain (Koch et 's, 1991; Pawson also Scott,Isabel Marant Wedge Sneakers, 1997). CAIR1/BAG3 was pulled down from unstimulated MDA435 cell lysates by the GSTPLCSH3 domain but not the PLCNSH2 or CSH2 internet domain names (frame 7a). consideration with EGF of the cells donating CAIR1/BAG3 did not alter CAIR1/BAG3 binding to the PLCSH3 domain, in comparison with what was observed with whole cell coimmunoprecipitation studies where EGF treatment reduced binding of CAIR1/BAG3 to PLC in whole cell lysates (parent 7b).
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